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KMID : 1141020100100020009
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Journal of Applied Oriental Medicine
2010 Volume.10 No. 2 p.9 ~ p.16
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Antioxidant Activity and Protective Effect on DNA Damage of Extracts from Ulmus devidiana var. japonica
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Ahn Joung-Jwa
Park Jae-Ho
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Abstract
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Objective: The present study was designed to examine the antioxidant activities and the protective effect on DNA damage of the extracts of Ulmus devidiana var, japonica.
Methods: The 1,1- diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl scavenging assay and Fe2+ chelating assay were performed for antioxidative effect and φX-174 RF I DNA cleavage assay and intracellular DNA damage assay were used for inhibitory effect of intracellular DNA damage.
Results: The EtOAC extracts at 200 ug/ml showed the highest acitivities of 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical and hydroxyl scavenging and Fe2+ chelating, which were 90.82, 65.86, and 52.42%, respectively. The slightly lower activities were found in hot-water with a similar pattern. The significant protective effect of DNA damage against oxidative stress was found from both EtOAC extracts and hot-water extracts. 3,5-bis(t-butyl) phenol, 1,3-benzenediol, Benzeneacetic acid, Aspidinol and Benzoic acid were identified by GC/MS analysis.
Conclusion: This study showed that the extracts of Ulmus devidiana var, japonica can be utilized as an effective antioxidants and protective effect of DNA damage against the oxidative stress.
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KEYWORD
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Ulmus devidiana, antioxidant activity, Fe2+ chelating, DNA damage
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